make
TCR
cloning software
Sample Name
Repertoire file
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Species
Human
Mouse
TCR Selection
Match List of Target TCRs
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Most Frequent TCRs
How many?
Randomly Sampled TCRs
How many?
Positive Controls (VDJdb)
TCR Replicates
Generate Synonymous CDR3 Variants
CDR3 Variants
Min. Hamming distance
Random seed
Shared (reproducible between runs)
Random (unique per run)
Constant Regions & Selection Cassettes
α/β region
Human + disulfide
Human + α/β domain switching
Human TRBC2 + disulfide
Mouse + disulfide
Human + disulfide-T2A-CD3ζ
Human + disulfide-T2A-puroR
Human + disulfide-T2A-staygold
Mouse + disulfide-T2A-puroR
Human
Mouse
γ/δ region
Human
Mouse
Show Advanced Options
Nested demultiplexing primer set
No two-step nested demultiplexing (max 1 plate)
Plate set 1
Plate set 2
Plate set 3
Plate set 4
Plate set 5
Plate set 6
Plate set 7
Plate set 8
Plate set 9
Plate set 10
Plate set 11
Plate set 12
Plate set 13
Plate set 14
Plate set 15
Plate set 16
Plate set 17
Array TCRs on plate type
384
96
T Cell Subset Selection
A/B cells
Include
Exclude
G/D cells
Include
Exclude
MAIT cells
Include
Exclude
CAIT cells
Include
Exclude
Resources
Example Input Files
Lab Protocols
Primer Sequences
Feature Annotations
Funders
makeTCR cloning software
makeTCR cloning software
makeTCR cloning software